Journal: Molecular Medicine Reports

Article Title: Transcriptional activation of ENPP2 by FoxO4 protects cardiomyocytes from doxorubicin-induced toxicity

doi: 10.3892/mmr.2021.12307

Figure Lengend Snippet: Effects of ENPP2 overexpression on DOX-induced autophagy in H9c2 cardiomyocytes. H9c2 cells were transfected with ENPP2 vector in order to obtain ENPP2-Oe cells. The transfection efficiency was detected via (A) western blotting and (B) reverse transcription-quantitative PCR. ***P<0.001 vs. Oe-NC. (C) ENPP2-Oe H9c2 cells were exposed to 5 µM DOX for 24 h and cell viability was measured. (D) The total reactive oxygen species production was assessed using a 2′,7′-dichlorodihydrofluorescein diacetate kit (magnification, ×200). ENPP2-Oe H9c2 cells were exposed to 5 µM DOX for 24 h, and subsequently (E) TBARS concentration levels and (F) GSH-Px expression levels were measured. (G) ENPP2-Oe H9c2 cells were exposed to 5 µM DOX for 24 h and the expression levels of the proteins involved in autophagy were evaluated. *P<0.05 and ***P<0.001 vs. control; # P<0.05, ## P<0.01 and ### P<0.001 vs. Oe-NC. DOX, doxorubicin; NC, negative control; TBARS, thiobarbituric acid reactive substances; GSH-Px, glutathione peroxidase; ENPP2, ectonucleotide pyrophosphatase/phosphodiesterase 2; Oe, overexpressing; ATG5, autophagy related 5.

Article Snippet: The increase in ROS levels was also examined using a commercially available thiobarbituric acid reactive substances (TBARS) assay kit (cat. no. 700870; Cayman Chemical Company) according to the manufacturer's instructions.

Techniques: Over Expression, Transfection, Plasmid Preparation, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction, Concentration Assay, Expressing, Control, Negative Control

Journal: Molecular Medicine Reports

Article Title: Transcriptional activation of ENPP2 by FoxO4 protects cardiomyocytes from doxorubicin-induced toxicity

doi: 10.3892/mmr.2021.12307

Figure Lengend Snippet: FoxO4 overexpression inhibits the effects of ENPP2 overexpression on DOX-induced autophagy in H9c2 cardiomyocytes. H9c2 cells that co-overexpressed FoxO4 and ENPP2 were exposed to 5 µM DOX for 24 h. Subsequently, (A) cell viability was measured with a MTT assay. (B) Total reactive oxygen species production was assessed using the DCFDA kit (magnification, ×200), (C) and the results were quantified. (D) TBARS concentration levels and (E) GSH-Px expression levels were detected using their corresponding kits. (F) The expression levels of the proteins involved in autophagy were evaluated via western blot analysis. *P<0.05, **P<0.01 and ***P<0.001. DOX, doxorubicin; TBARS, thiobarbituric acid reactive substances; GSH-Px, glutathione peroxidase; ENPP2, ectonucleotide pyrophosphatase/phosphodiesterase 2; Oe, overexpressing; NC, negative control; ATG5, autophagy related 5.

Article Snippet: The increase in ROS levels was also examined using a commercially available thiobarbituric acid reactive substances (TBARS) assay kit (cat. no. 700870; Cayman Chemical Company) according to the manufacturer's instructions.

Techniques: Over Expression, MTT Assay, Concentration Assay, Expressing, Western Blot, Negative Control

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: Renal Dopamine Oxidation and Inflammation in High Salt Fed Rats

doi: 10.1161/JAHA.119.014977

Figure Lengend Snippet: Food and Water Intake and Oxidative Stress Markers

Article Snippet: An 8‐isoprostane ELISA kit (Cat. No. 516351) and malondialdehyde colorimetric/fluorometric kit (Cat. No. 700870) were purchased from Cayman Chemicals (Ann Arbor, MI).

Techniques:

Journal: Antioxidants

Article Title: Nutraceutical Potential of Djulis ( Chenopodium formosanum ) Hull: Phytochemicals, Antioxidant Activity, and Liver Protection

doi: 10.3390/antiox13060721

Figure Lengend Snippet: Effects of Djulis hull and kernel crude extracts on the activities of liver antioxidant enzymes, including ( A ) SOD, ( B ) GPx, and ( C ) CAT, as well as the ( D ) TBARS content of mice in LPS-induced acute liver injury. SOD, superoxide dismutase; GPx, glutathione peroxidase; CAT, catalase; TBARS, thiobarbituric acid reactive substances. A one-tailed Mann-Whitney U test was used for statistical analysis. Values are represented as the mean ± SEM ( n = 6), where # p < 0.05 and ## p < 0.01 compared with the control group, and * p < 0.05 and ** p < 0.01 compared with the water group.

Article Snippet: The antioxidant enzyme activities of glutathione peroxidase (GPx) (703102, Cayman Chemical, Ann Arbor, MI, USA) and catalase (CAT) (707002, Cayman Chemical) and the lipid peroxidative index of thiobarbituric acid reactive substances (TBARS) (700870, Cayman Chemical) were measured using the Cayman assay kit.

Techniques: One-tailed Test, MANN-WHITNEY, Control